"A rejuvenated Hand, seeking clan secrets...a powerful being on the hunt for a tailed beast or two...S.H.I.E.L.D receives the aid of a powerful trio to deal with this threat." — Chapter Briefing
The only way to unlock Jiraiya and Orochimaru is to complete the list of Tasks.
One of the Tasks requires the defeat of an Epic Boss. This can be done by having the requirements needed to unlock the Epic Boss or by skipping the task by paying 30 Gold.
The requirements are:
In this Special Operations, the Hand receives new members, namely members of the Akatsuki and other such ninjas. In Mission 1, the Hand reappears in Hell’s Kitchen where it appears as if they are looking for something. Elektra and Daredevil engage Lady Bullseye and Ikari respectively, both of whom appear to be leading the search. The whole operation is found to be led by Orochimaru and Kabuto, who are faced by Orochimaru's old comrades, Jiraiya and Tsunade. After the battle, both escape. Jiraiya then finds his former student, Konan, and he fights her, thinking she is with the Akatsuki. After the battle, Konan reveals that she intends to defect from the group since she feels that the Akatsuki she had helped her friends to co-find has been perverted into a criminal organization and joining the Hand was the last straw. Orochimaru also approaches S.H.I.E.L.D and states his intention to join since the Hand as no longer anything to offer him.
A strange figure, Kinshiki Ōtsutsuki, appears in search of Naruto, the vessel of the Nine-tailed Fox and S.H.I.E.L.D. fights him off.
In Mission 2, Orochimaru reveals that the Hand intends to look for and harvest the latent abilities of special clans i.e the Hyuga clan’s Byakugan, the Yamanaka clan’s Mind Transfer abilities. Hand ninjas find and go after the ninjas who must fight their pursuers while Konan is cornered by the Silver Samurai. At the end of the mission, Gengo and Gorgon is revealed to be leading the Hand assault and in the battle, they let slip that the clan abilities aren’t really their target after all and in fact, it is the Tailed Beast as well.
In Mission 3, all of the Akatsuki members who joined the Hand reveal themselves and launch an all-out assault. The ninjas quickly respond to protect their friend, who is greatly weakened by his fight with Kinshiki Ōtsutsuki (Via deploy in Mission 2). Each of the Akatsuki members, Kabuto and the members of the Hand are fought as mini-bosses. At the end of the battle, Konan confronts Obito and Nobu who are the ringleaders of the Hand’s scheme. After defeating them, the three Sannin step up to face Kinshiki once more.